Cellular localization of 2-[3H]deoxy-D-glucose from paraffin-embedded brains
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منابع مشابه
Cellular localization of 2-[3H]deoxy-D-glucose from paraffin-embedded brains.
Results of experiments in which regional neuronal activity is revealed by a 2-[3H]deoxy-D-glucose (3H-2-DG)-paraffin section-emulsion autoradiography method are described. The trigeminal pathway of freely behaving mice was activated differentially by selective patterns of whisker removal. One hour after injection of concentrated 3H-2-DG, the animals were perfused systemically with a periodate/l...
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obtained without prior incubation with the ABTS reagent (Figure 2). Consequently, both quantitative levels and cellular distribution data can be readily obtained from the same cells in this assay. Presumably, this assay can be extended to monitor the expression, conformational change, or posttranslational modification of any protein for which an appropriate antiserum exists. Obviously, it is ne...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1981
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.01-05-00519.1981